Typing and subtyping clinical isolates of influenza virus using reverse transcription-polymerase chain reaction
Identifieur interne : 001C05 ( Main/Exploration ); précédent : 001C04; suivant : 001C06Typing and subtyping clinical isolates of influenza virus using reverse transcription-polymerase chain reaction
Auteurs : Robert L. Atmar [États-Unis] ; Barbara D. Baxter [États-Unis]Source :
- Clinical and Diagnostic Virology [ 0928-0197 ] ; 1996.
English descriptors
- Teeft :
- Ahah3 primers, Amplification, Antigenic, Antigenic similarity, Antigenic variants, Assay, Atmar, Base pairs, Baxter, Baylor college, Baylor plaza, Cdna synthesis, Chain reaction assay, Clin, Clinical samples, Clinical specimens, Deoxynucleoside triphosphates, Diagnostic virology, Disease control, Elsevier science, Enzyme immunoassay, Ethidium bromide, Harvest fluids, Hemagglutinin, Hemagglutinin gene, Hybridization, Influenza, Influenza virus, Influenza virus products, Influenza viruses, Laboratory strains, Magnesium chloride, Matrix gene, Microbiol, Molecular weight markers, National institutes, Negative reagent control, Negative reagent controls, Nucleic acids, Polymerase, Polymerase chain reaction, Potassium chloride, Primer, Reagent, Reference strain, Reference strains, Research center, Respective viruses, Respiratory secretions, Same positions, Serial dilutions, Slot blot hybridization, Subtypes, Tris hydrochloride, Virology, Virus, Virus detection limits, Virus pools.
Abstract
Abstract: Background: Influenza virus infections are a major cause of morbidity and the identification of the type or subtype of a clinical isolate has important clinical and epidemiological implications. Objectives: To evaluate the ability of a reverse transcription-polymerase chain reaction (RT-PCR) assay to type and subtype clinical human isolates of influenza virus. Study design: Reference strains of influenza A H1N1, A H3N2, and B viruses and human clinical isolates of influenza virus representing antigenic variants from the last 15 years were evaluated using an RT-PCR assay. Results: Amplicons of 325, 198 and 365 base pairs in length were obtained from RNA extracted from influenza A H1N1, A H3N2 and B viruses, respectively. All human-derived A H1N1, A H3N2, and B reference strains and antigenic variants tested were correctly identified. Conclusions: RT-PCR is an effective alternative to traditional methods for typing and subtyping influenza viruses.
Url:
DOI: 10.1016/S0928-0197(96)00254-1
Affiliations:
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Objectives: To evaluate the ability of a reverse transcription-polymerase chain reaction (RT-PCR) assay to type and subtype clinical human isolates of influenza virus. Study design: Reference strains of influenza A H1N1, A H3N2, and B viruses and human clinical isolates of influenza virus representing antigenic variants from the last 15 years were evaluated using an RT-PCR assay. Results: Amplicons of 325, 198 and 365 base pairs in length were obtained from RNA extracted from influenza A H1N1, A H3N2 and B viruses, respectively. All human-derived A H1N1, A H3N2, and B reference strains and antigenic variants tested were correctly identified. Conclusions: RT-PCR is an effective alternative to traditional methods for typing and subtyping influenza viruses.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region></list><tree><country name="États-Unis"><noRegion><name sortKey="Atmar, Robert L" sort="Atmar, Robert L" uniqKey="Atmar R" first="Robert L." last="Atmar">Robert L. Atmar</name></noRegion><name sortKey="Atmar, Robert L" sort="Atmar, Robert L" uniqKey="Atmar R" first="Robert L." last="Atmar">Robert L. Atmar</name><name sortKey="Baxter, Barbara D" sort="Baxter, Barbara D" uniqKey="Baxter B" first="Barbara D." last="Baxter">Barbara D. Baxter</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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